The dynamic clamp is actually an electrophysiology method which makes use of the real-time interface between on or various living cells and also a computer or analog device in order to simulate the dynamic processes like the membrane or the synaptic currents in the living cells.
The digital clamp is going to provide the neuroscientists with such new kind of platform in order to conduct ion channel experiments. This is certainly capable of performing the traditional voltage clamp and also the current clamp, the dynamic clamp would bridge the two neurons, the single-electrode voltage clamp would use the novel software-switched method as well as sophisticated control algorithms like the detect-then-trigger control as well as intermittent voltage clamp. These capabilities in one single instrument opens up that world of new experiments which are possible in the lab.
The dynamic clamp is that such first clamping amplifier that is available with the quick digital feedback implemented with that front-end digital signal processor without depending on the host PC. The digital signal processors have that only recently become quick enough to fulfill such function in real time. This is one all-digital device, this comes with capabilities which are not possible with the use of standard analog feedback voltage clamps. Such dynamic clamping at a quick rate and also single electrode intermittent clamping. The modules would communicate through such Ethernet network. The number of its headstages is really scalable in order to satisfy such requirements for specific electrophysiological experiments. Such Ethernet connector can be daisy chained to different units controlled from a single host computer.
Well, the dynamic clamp technique was actually first launched in the year 1993 being a method to simulate the ionic conductances in the neurons on the patch clamp recordings. This is a kind of method that typically included recording the potential of the cell membrane and also in the transmission of data to the computer, calculating such appropriate current which is going to be injected into the neuron and also sending such value back to the amplifier. The communication between the computer as well as the amplifier would limit the rate in which the dynamic clamp calculations may be updated.
Also, the additional hardware and also software required to do dynamic clamp has really limited the application. Such all-in-one solution is now being made available as one free upgrade to the software and also the amplifier for those researchers who are actually interested in conducting dynamic clamp experiments.
The simulation of such population of channels in the neuronal membrane includes modeling the kinetics of the gating mechanisms of the channel. This channel kinetics may either be modeled with the use of multiple independent gates. Those values which are obtained from such gating equations are then being utilized to compute the current applied to the neuron. The calculation of such current may either be defined according to conductance or by permeability. For those conductance models, the value that is defined from the gating calculations is then combined with that reversal potential of the ions which pass through those simulated channels and the conductance of the simulated channels.